Deterministic separation of cancer cells from blood at 10 mL/min.

Citation:

Loutherback K1, D'Silva J, Liu Wu Austin RH Sturm JC. LA. “Deterministic separation of cancer cells from blood at 10 mL/min..” AIP Adv. 2012 Dec;2(4):42107. Epub 2012 Oct 3. (2012).

Abstract:

Circulating tumor cells (CTCs) and circulating clusters of cancer and stromal cells have been identified in the blood of patients with malignant cancer and can be used as a diagnostic for disease severity, assess the efficacy of different treatment strategies and possibly determine the eventual location of metastatic invasions for possible treatment. There is thus a critical need to isolate, propagate and characterize viable CTCs and clusters of cancer cells with their associated stroma cells. Here, we present a microfluidic device for mL/min flow rate, continuous-flow capture of viable CTCs from blood using deterministic lateral displacement (DLD) arrays. We show here that a DLD array device can isolate CTCs from blood with capture efficiency greater than 85% CTCs at volumetric flow rates of up to 10 mL/min with no effect on cell viability.

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Last updated on 02/24/2016